mouse pre osteoblast cell line mc3t3 e1 Search Results


mc3t3-e1 subclone 4  (ATCC)
99
ATCC mc3t3-e1 subclone 4
Mc3t3 E1 Subclone 4, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
mc3t3-e1 subclone 4 - by Bioz Stars, 2026-03
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mouse osteoblast cell line mc3t3-e1  (ScienCell)
90
ScienCell mouse osteoblast cell line mc3t3-e1
(A) The cell viability of <t>MC3T3-E1</t> after 200 ng/mL BMP2 treatment for 0, 12, 24, 36, and 48 h was assessed using CCK-8 assay. (B) The number of calcium nodules was counted in 200 ng/mL BMP2-treated MC3T3-E1 cells by alizarin red staining, compared with untreated MC3T3-E1 group. (C) On days 0, 7, and 14, the mRNA levels of the <t>osteoblast</t> marker genes OPN, Col1A1, and Runx2 were assessed using by RT-PCR in induced MC3T3-E1 cells. (D) Alkaline phosphatase (ALP) activity in BMP2-treated MC3T3-E1 cells on days 0, 7 and 14 was measured using a commercial assay kit. (E) RT-PCR analysis of the expression of miR-452-3p in induced MC3T3-E1 cells. N = 3, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Mouse Osteoblast Cell Line Mc3t3 E1, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse osteoblast cell line mc3t3-e1/product/ScienCell
Average 90 stars, based on 1 article reviews
mouse osteoblast cell line mc3t3-e1 - by Bioz Stars, 2026-03
90/100 stars
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mouse osteoblast cell line mc3t3-e1  (Chongqing Key)
90
Chongqing Key mouse osteoblast cell line mc3t3-e1
(A) The cell viability of <t>MC3T3-E1</t> after 200 ng/mL BMP2 treatment for 0, 12, 24, 36, and 48 h was assessed using CCK-8 assay. (B) The number of calcium nodules was counted in 200 ng/mL BMP2-treated MC3T3-E1 cells by alizarin red staining, compared with untreated MC3T3-E1 group. (C) On days 0, 7, and 14, the mRNA levels of the <t>osteoblast</t> marker genes OPN, Col1A1, and Runx2 were assessed using by RT-PCR in induced MC3T3-E1 cells. (D) Alkaline phosphatase (ALP) activity in BMP2-treated MC3T3-E1 cells on days 0, 7 and 14 was measured using a commercial assay kit. (E) RT-PCR analysis of the expression of miR-452-3p in induced MC3T3-E1 cells. N = 3, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Mouse Osteoblast Cell Line Mc3t3 E1, supplied by Chongqing Key, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse osteoblast cell line mc3t3-e1/product/Chongqing Key
Average 90 stars, based on 1 article reviews
mouse osteoblast cell line mc3t3-e1 - by Bioz Stars, 2026-03
90/100 stars
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mouse osteoblastic cell line mc3t3-e1  (Flow Laboratories)
90
Flow Laboratories mouse osteoblastic cell line mc3t3-e1
(A) The cell viability of <t>MC3T3-E1</t> after 200 ng/mL BMP2 treatment for 0, 12, 24, 36, and 48 h was assessed using CCK-8 assay. (B) The number of calcium nodules was counted in 200 ng/mL BMP2-treated MC3T3-E1 cells by alizarin red staining, compared with untreated MC3T3-E1 group. (C) On days 0, 7, and 14, the mRNA levels of the <t>osteoblast</t> marker genes OPN, Col1A1, and Runx2 were assessed using by RT-PCR in induced MC3T3-E1 cells. (D) Alkaline phosphatase (ALP) activity in BMP2-treated MC3T3-E1 cells on days 0, 7 and 14 was measured using a commercial assay kit. (E) RT-PCR analysis of the expression of miR-452-3p in induced MC3T3-E1 cells. N = 3, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Mouse Osteoblastic Cell Line Mc3t3 E1, supplied by Flow Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse osteoblastic cell line mc3t3-e1/product/Flow Laboratories
Average 90 stars, based on 1 article reviews
mouse osteoblastic cell line mc3t3-e1 - by Bioz Stars, 2026-03
90/100 stars
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mc3t3-e1 subclone 14  (ATCC)
96
ATCC mc3t3-e1 subclone 14
(A) The cell viability of <t>MC3T3-E1</t> after 200 ng/mL BMP2 treatment for 0, 12, 24, 36, and 48 h was assessed using CCK-8 assay. (B) The number of calcium nodules was counted in 200 ng/mL BMP2-treated MC3T3-E1 cells by alizarin red staining, compared with untreated MC3T3-E1 group. (C) On days 0, 7, and 14, the mRNA levels of the <t>osteoblast</t> marker genes OPN, Col1A1, and Runx2 were assessed using by RT-PCR in induced MC3T3-E1 cells. (D) Alkaline phosphatase (ALP) activity in BMP2-treated MC3T3-E1 cells on days 0, 7 and 14 was measured using a commercial assay kit. (E) RT-PCR analysis of the expression of miR-452-3p in induced MC3T3-E1 cells. N = 3, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Mc3t3 E1 Subclone 14, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mc3t3-e1 subclone 14/product/ATCC
Average 96 stars, based on 1 article reviews
mc3t3-e1 subclone 14 - by Bioz Stars, 2026-03
96/100 stars
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mouse osteoblastic cell line mc3t3-e1  (Mediatech)
90
Mediatech mouse osteoblastic cell line mc3t3-e1
(A) The cell viability of <t>MC3T3-E1</t> after 200 ng/mL BMP2 treatment for 0, 12, 24, 36, and 48 h was assessed using CCK-8 assay. (B) The number of calcium nodules was counted in 200 ng/mL BMP2-treated MC3T3-E1 cells by alizarin red staining, compared with untreated MC3T3-E1 group. (C) On days 0, 7, and 14, the mRNA levels of the <t>osteoblast</t> marker genes OPN, Col1A1, and Runx2 were assessed using by RT-PCR in induced MC3T3-E1 cells. (D) Alkaline phosphatase (ALP) activity in BMP2-treated MC3T3-E1 cells on days 0, 7 and 14 was measured using a commercial assay kit. (E) RT-PCR analysis of the expression of miR-452-3p in induced MC3T3-E1 cells. N = 3, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Mouse Osteoblastic Cell Line Mc3t3 E1, supplied by Mediatech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse osteoblastic cell line mc3t3-e1/product/Mediatech
Average 90 stars, based on 1 article reviews
mouse osteoblastic cell line mc3t3-e1 - by Bioz Stars, 2026-03
90/100 stars
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mouse mc3t3-e1 osteoblast cell line  (Amgen)
90
Amgen mouse mc3t3-e1 osteoblast cell line
(A) The cell viability of <t>MC3T3-E1</t> after 200 ng/mL BMP2 treatment for 0, 12, 24, 36, and 48 h was assessed using CCK-8 assay. (B) The number of calcium nodules was counted in 200 ng/mL BMP2-treated MC3T3-E1 cells by alizarin red staining, compared with untreated MC3T3-E1 group. (C) On days 0, 7, and 14, the mRNA levels of the <t>osteoblast</t> marker genes OPN, Col1A1, and Runx2 were assessed using by RT-PCR in induced MC3T3-E1 cells. (D) Alkaline phosphatase (ALP) activity in BMP2-treated MC3T3-E1 cells on days 0, 7 and 14 was measured using a commercial assay kit. (E) RT-PCR analysis of the expression of miR-452-3p in induced MC3T3-E1 cells. N = 3, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Mouse Mc3t3 E1 Osteoblast Cell Line, supplied by Amgen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse mc3t3-e1 osteoblast cell line/product/Amgen
Average 90 stars, based on 1 article reviews
mouse mc3t3-e1 osteoblast cell line - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


(A) The cell viability of MC3T3-E1 after 200 ng/mL BMP2 treatment for 0, 12, 24, 36, and 48 h was assessed using CCK-8 assay. (B) The number of calcium nodules was counted in 200 ng/mL BMP2-treated MC3T3-E1 cells by alizarin red staining, compared with untreated MC3T3-E1 group. (C) On days 0, 7, and 14, the mRNA levels of the osteoblast marker genes OPN, Col1A1, and Runx2 were assessed using by RT-PCR in induced MC3T3-E1 cells. (D) Alkaline phosphatase (ALP) activity in BMP2-treated MC3T3-E1 cells on days 0, 7 and 14 was measured using a commercial assay kit. (E) RT-PCR analysis of the expression of miR-452-3p in induced MC3T3-E1 cells. N = 3, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Journal: PeerJ

Article Title: miR-452-3p inhibited osteoblast differentiation by targeting Smad4

doi: 10.7717/peerj.12228

Figure Lengend Snippet: (A) The cell viability of MC3T3-E1 after 200 ng/mL BMP2 treatment for 0, 12, 24, 36, and 48 h was assessed using CCK-8 assay. (B) The number of calcium nodules was counted in 200 ng/mL BMP2-treated MC3T3-E1 cells by alizarin red staining, compared with untreated MC3T3-E1 group. (C) On days 0, 7, and 14, the mRNA levels of the osteoblast marker genes OPN, Col1A1, and Runx2 were assessed using by RT-PCR in induced MC3T3-E1 cells. (D) Alkaline phosphatase (ALP) activity in BMP2-treated MC3T3-E1 cells on days 0, 7 and 14 was measured using a commercial assay kit. (E) RT-PCR analysis of the expression of miR-452-3p in induced MC3T3-E1 cells. N = 3, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: The mouse osteoblast cell line (MC3T3-E1) was purchased from ScienCell (Carlsbad, CA, USA) and cultured in alpha-minimum essential medium (α-MEM; SH30265.01B; Hyclone, South Logan, UT, USA) containing 1% penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO, USA), 10% foetal bovine serum (FBS).

Techniques: CCK-8 Assay, Staining, Marker, Reverse Transcription Polymerase Chain Reaction, Activity Assay, Expressing